A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.
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机译：已开发出一种单次使用的一次性碱性磷酸酶（ALP）生物传感器。它基于对ALP酶促反应产生的苯酚的检测。它可以在pH 10的介质中在25°C下运行。测量的ALP为0–300 IU / L。不受干扰的葡萄糖，抗坏血酸和尿素的允许浓度分别为10 mM / L，5 mg / L和400 mg / L。将实验结果与通过分光光度法在牛血清中获得的结果进行比较。生物传感器输出和ALP浓度之间存在出色的线性关系。该生物传感器和分光光度计的测量结果之间的一致性也很出色。
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